Nano Archive

New antibodies immobilization system into a graphite-polysulfone membrane for amperometric immunosensors

Sanchez Ordonez, Samuel and Fabregas, Esteve (2007) New antibodies immobilization system into a graphite-polysulfone membrane for amperometric immunosensors. BIOSENSORS & BIOELECTRONICS, 22 (6). pp. 965-972. ISSN 0956-5663

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Official URL: http://www.sciencedirect.com/science?_ob=ArticleUR...

Abstract

Polysulfone membrane is used for the first time for the preparation of electrochemical immunosensors. A disposable immunosensor based on a porous conductor polymer graphite-polysulfone-electrode has been developed using a phase inversion technique for the determination of anti-rabbit IgG (anti-RIgG) as a model analyte. To construct the sensor, a conductor membrane was deposited on the surface of working graphite-epoxy composite (GEC) electrode. The membrane was characterized by SEM. This sensor was based on the competitive assay between free and labeled anti-RIgG for the available binding sites of immobilized rabbit IgG (RIgG). Incubation parameters were optimized in this work. The immunological reaction was detected using an enzymatic -labeling procedure (HRP enzyme) combined with the amperometric detection using H2O2 as substrate and hydroquinone as mediator. This sensor shows stability during a week and a good reproducibility. The current was monitored amperometrically at -0.1 V versus SCE and this method showed a linear range of the anti-RIgG from 1 to 6 mu g/ml. The detection limit was determined to be 0.77 mu g/ml. (c) 2006 Elsevier B.V. All rights reserved.

Item Type:Article
Uncontrolled Keywords:polysulfone membrane; immunosensor; amperometric biosensor; antibody immobilization; biocomposite; composite
Subjects:Biomedical Science > Nanobiotechnology
ID Code:2182
Deposited By:M T V
Deposited On:24 Dec 2008 14:51
Last Modified:24 Dec 2008 14:51

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