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A label-free electrochemical immunoassay for carcinoembryonic antigen (CEA) based on gold nanoparticles (AuNPs) and nonconductive polymer film

Tang, Hao and Chen, Jinhua and Nie, Lihua and Kuang, Yafei and Yao, Shouzhuo (2007) A label-free electrochemical immunoassay for carcinoembryonic antigen (CEA) based on gold nanoparticles (AuNPs) and nonconductive polymer film. BIOSENSORS & BIOELECTRONICS, 22 (6). pp. 1061-1067. ISSN 0956-5663

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Official URL: http://www.sciencedirect.com/science?_ob=ArticleUR...

Abstract

A simple and sensitive label-free electrochemical immunoassay electrode for detection of carcinoembryonic antigen (CEA) has been developed. CEA antibody (CEAAb) was covalently attached on glutathione (GSH) monolayer-modified gold nanoparticle (AuNP) and the resulting CEAAb-AuNP bioconjugates were immobilized on Au electrode by electro-copolymerization with o-aminophenol (OAP). Electrochemical impedance spectroscopy and cyclic voltammetry studies demonstrate that the formation of CEA antibody-antigen complexes increases the electron transfer resistance of [Fe(CN)(6)](3-/4-) redox pair at the poly-OAP/CEAAb-AuNP/Au electrode. The use of CEA antibody-AuNP bioconjugates and poly-OAP film could enhance the sensitivity and anti-non specific binding of the resulting immunoassay electrode. The preliminary application of poly-OAP/CEAAb-AuNP/Au electrode for detection of CEA was also evaluated. (c) 2006 Elsevier B.V. All rights reserved.

Item Type:Article
Uncontrolled Keywords:gold nanoparticles; poly-o-aminophenol film; electrochemical impedance spectroscopy; immunoassay; carcinoembryonic antigen
Subjects:Biomedical Science > Nanomedicine
ID Code:2170
Deposited By:M T V
Deposited On:02 Jan 2009 11:05
Last Modified:02 Jan 2009 11:05

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