Tang, Hao and Chen, Jinhua and Nie, Lihua and Kuang, Yafei and Yao, Shouzhuo (2007) A label-free electrochemical immunoassay for carcinoembryonic antigen (CEA) based on gold nanoparticles (AuNPs) and nonconductive polymer film. BIOSENSORS & BIOELECTRONICS, 22 (6). pp. 1061-1067. ISSN 0956-5663
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A simple and sensitive label-free electrochemical immunoassay electrode for detection of carcinoembryonic antigen (CEA) has been developed. CEA antibody (CEAAb) was covalently attached on glutathione (GSH) monolayer-modified gold nanoparticle (AuNP) and the resulting CEAAb-AuNP bioconjugates were immobilized on Au electrode by electro-copolymerization with o-aminophenol (OAP). Electrochemical impedance spectroscopy and cyclic voltammetry studies demonstrate that the formation of CEA antibody-antigen complexes increases the electron transfer resistance of [Fe(CN)(6)](3-/4-) redox pair at the poly-OAP/CEAAb-AuNP/Au electrode. The use of CEA antibody-AuNP bioconjugates and poly-OAP film could enhance the sensitivity and anti-non specific binding of the resulting immunoassay electrode. The preliminary application of poly-OAP/CEAAb-AuNP/Au electrode for detection of CEA was also evaluated. (c) 2006 Elsevier B.V. All rights reserved.
|Uncontrolled Keywords:||gold nanoparticles; poly-o-aminophenol film; electrochemical impedance spectroscopy; immunoassay; carcinoembryonic antigen|
|Subjects:||Biomedical Science > Nanomedicine|
|Deposited By:||M T V|
|Deposited On:||02 Jan 2009 11:05|
|Last Modified:||02 Jan 2009 11:05|
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