Nano Archive

Nanostructured polyelectrolyte films for engineering highly sensitive tyrosinase biosensors: Specifics of enzyme-polyelectrolyte structures

Dubacheva, G. V. and Porus, M. V. and Kurochkin, I. N. and Sigolaeva, L. V. and Pergushov, D. V. (2008) Nanostructured polyelectrolyte films for engineering highly sensitive tyrosinase biosensors: Specifics of enzyme-polyelectrolyte structures. Nanotechnologies in Russia, Volume 3 (3-4). pp. 221-227.

Full text is not hosted in this archive but may be available via the Official URL, or by requesting a copy from the corresponding author.

Official URL: http://www.springerlink.com/content/p8277425w88723...

Abstract

The correlation was studied between the activity of tyrosinase electrodes engineered by layer-by-layer deposition technology and the number of PDDA/tyrosinase layers (where PDDA stands for poly(diallyldimethylammonium chloride)). The last deposited layer is the active one regardless of the number of layers. Atomic force microscopy (AFM) characterization of the topology of (PDDA/tyrosinase)1 and (PDDA/tyrosinase)2 films showed that these films have identical structures. The operational stability of tyrosinase biosensors coated with (PDDA/tyrosinase)1 films was examined. Various methods for stabilizing tyrosinase biosensors were tested. Crosslinking with glutaraldehyde (GA) improved 2.5-fold the operational stability of (PDDA/tyrosinase)1 films.

Item Type:Article
Subjects:Physical Science > Nano objects
Material Science > Nanochemistry
ID Code:2094
Deposited By:Prof. Alexey Ivanov
Deposited On:19 Dec 2008 13:15
Last Modified:01 Feb 2009 20:35

Repository Staff Only: item control page