Dubacheva, G. V. and Porus, M. V. and Kurochkin, I. N. and Sigolaeva, L. V. and Pergushov, D. V. (2008) Nanostructured polyelectrolyte films for engineering highly sensitive tyrosinase biosensors: Specifics of enzyme-polyelectrolyte structures. Nanotechnologies in Russia, Volume 3 (3-4). pp. 221-227.
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Official URL: http://www.springerlink.com/content/p8277425w88723...
The correlation was studied between the activity of tyrosinase electrodes engineered by layer-by-layer deposition technology and the number of PDDA/tyrosinase layers (where PDDA stands for poly(diallyldimethylammonium chloride)). The last deposited layer is the active one regardless of the number of layers. Atomic force microscopy (AFM) characterization of the topology of (PDDA/tyrosinase)1 and (PDDA/tyrosinase)2 films showed that these films have identical structures. The operational stability of tyrosinase biosensors coated with (PDDA/tyrosinase)1 films was examined. Various methods for stabilizing tyrosinase biosensors were tested. Crosslinking with glutaraldehyde (GA) improved 2.5-fold the operational stability of (PDDA/tyrosinase)1 films.
|Subjects:||Physical Science > Nano objects|
Material Science > Nanochemistry
|Deposited By:||Prof. Alexey Ivanov|
|Deposited On:||19 Dec 2008 13:15|
|Last Modified:||01 Feb 2009 20:35|
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