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Photostability of thiol-capped CdTe quantum dots in living cells: the effect of photo-oxidation

Ma, J and Chen, Ji-Yao and Guo, J and Wang, CC and Yang, WL and Xu, L and Wang, PN (2006) Photostability of thiol-capped CdTe quantum dots in living cells: the effect of photo-oxidation. Nanotechnology, 17 (9). pp. 2083-2089. ISSN 09574484

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Official URL: http://dx.doi.org/10.1088/0957-4484/17/9/002

Abstract

The photostability of thiol-capped CdTe quantum dots (QDs) in Euglena gracilis (EG 277) and human embryonic kidney (HEK 293) cells was studied. The photobleaching for the cellular QDs is dependent both on the irradiation power density and the QD local concentration. The photostability of cellular QDs is better than that of chlorophyll in EG 277 cells and of green fluorescence protein (GFP) in HEK 293 cells, and is much better than that of FITC when the local concentration of QDs is not too low. The photobleaching of cellular QDs was remarkably reduced in the nitrogen treated EG 277 cells, indicating that photobleaching in living cells mainly results from photo-oxidation. The effect of photo-oxidation on QD photobleaching was further confirmed by comparing the situations in oxygen treated and nitrogen treated QD aqueous solutions. The photobleaching rate is related to the irradiation power density and the local density of QDs. The higher irradiation power density and oxygen abundance and lower QD concentration will result in a higher photobleaching rate.

Item Type:Article
Subjects:Analytical Science > Microscopy and probe methods
Physical Science > Quantum phenomena
ID Code:202
Deposited By:Lesley Tobin
Deposited On:17 Nov 2008 16:07
Last Modified:30 Jan 2009 16:16

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