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Enhanced multi-spectral imaging of live breast cancer cells using immunotargeted gold nanoshells and two-photon excitation microscopy

Bickford, Lissett and Sun, Jiantang and Fu, Kun and Lewinski, Nastassja and Nammalvar, Vengadesan and Chang, Joseph and Drezek, Rebekah (2008) Enhanced multi-spectral imaging of live breast cancer cells using immunotargeted gold nanoshells and two-photon excitation microscopy. NANOTECHNOLOGY, 19 (31).

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Official URL: http://dx.doi.org/10.1088/0957-4484/19/31/315102

Abstract

We demonstrate the capability of using immunotargeted gold nanoshells as contrast agents for in vitro two-photon microscopy. The two-photon luminescence properties of different-sized gold nanoshells are first validated using near-infrared excitation at 780 nm. The utility of two-photon microscopy as a tool for imaging live HER2-overexpressing breast cancer cells labeled with anti-HER2-conjugated nanoshells is then explored and imaging results are compared to normal breast cells. Five different imaging channels are simultaneously examined within the emission wavelength range of 451-644 nm. Our results indicate that under near-infrared excitation, superior contrast of SK-BR-3 cancer cells labeled with immunotargeted nanoshells occurs at an emission wavelength ranging from 590 to 644 nm. Luminescence from labeled normal breast cells and autofluorescence from unlabeled cancer and normal cells remain imperceptible under the same conditions.

Item Type:Article
Subjects:Analytical Science > Microscopy and probe methods
Biomedical Science > Nanomedicine
ID Code:1874
Deposited By:Farnush Anwar
Deposited On:16 Dec 2008 11:10
Last Modified:16 Dec 2008 11:12

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