Kilian, Kristopher A. and Boecking, Till and Gaus, Katharina and Gal, Michael and Gooding, J. Justin (2007) Peptide-modified optical filters for detecting protease activity. ACS NANO, 1 (4). pp. 355-361.
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Official URL: http://pubs.acs.org/doi/abs/10.1021/nn700141n
The organic derivatization of silicon-based nanoporous photonic crystals is presented as a method to immobilize peptides for the detection of protease enzymes in solution. A narrow-line-width rugate filter, a one-dimensional photonic crystal, is fabricated that exhibits a high-reflectivity optical resonance that is sensitive to small changes in the refractive index at the pore walls. To immobilize peptide in the pore of the photonic crystal, the hydrogen-terminated silicon surface was first modified with the alkene 10-succinimidyl undecenoate via hydrosilylation. The monolayer with the succinimide ester moiety at the distal end served the dual function of protecting the underlying silicon from oxidation as well as providing a surface suitable for subsequent derivatization with amines. The surface was further modified with 1-aminohexa(ethylene glycol) (EG(6)) to resist nonspecific adsorption of proteins common in complex biological samples. The distal hydroxyl of the EG(6) is activated using the solid-phase coupling reagent disuccinimidyl carbonate for selective immobilization of peptides as protease recognition elements. X-ray photoelectron spectroscopy analysis reveals high activation and coupling efficiency at each stage of the functionalization. Exposure of the peptide-modified crystals to the protease subtilisin in solution causes a change in the refractive index, resulting in a shift of the resonance to shorter wavelengths, indicating cleavage of organic material within the pores. The lowest detected concentration of enzyme was 37 nM (7.4 pmol in 200 mu L).
|Uncontrolled Keywords:||porous silicon; biosensor; photonic crystal; rugate filter; hydrosilylation; protease|
|Subjects:||Material Science > Functional and hybrid materials|
Material Science > Nanofabrication processes and tools
Physical Science > Photonics
|Deposited By:||Farnush Anwar|
|Deposited On:||16 Dec 2008 11:52|
|Last Modified:||16 Dec 2008 11:52|
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