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Imaging of biomaterials in liquids: a comparison between conventional and Q-controlled amplitude modulation ('tapping mode') atomic force microscopy

Ebeling, D and Holscher, H and Fuchs, Harald and Anczykowski, B and Schwarz, U. D. (2006) Imaging of biomaterials in liquids: a comparison between conventional and Q-controlled amplitude modulation ('tapping mode') atomic force microscopy. Nanotechnology, 17 (7). S221-S226. ISSN 09574484

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Official URL: http://dx.doi.org/10.1088/0957-4484/17/7/S20

Abstract

Lambda phage DNA and DPPC thin films are imaged in liquids by atomic force microscopy applying the amplitude modulation mode (‘tapping mode’)with active enhancement of the Q-factor by a ‘Q-control’ electronics. The topography of the resulting images is compared with images obtained without active Q-control. To enable a meaningful comparison, individual scan lines are alternately recorded with and without Q-factor enhancement using scan parameters optimized for each mode separately. As the major finding, significant height differences of topographical features are observed between the two modes. The heights measured with active Q-control are reproducibly higher compared to the ones observed without Q enhancement. This effect is attributed to the reduction of tip–sample forces by Q-control.

Item Type:Article
Subjects:Analytical Science > Microscopy and probe methods
Material Science > Bio materials
ID Code:175
Deposited By:Lesley Tobin
Deposited On:12 Nov 2008 16:49
Last Modified:02 Mar 2009 11:11

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