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Zinc oxide nanocrystals for nonresonant nonlinear optical microscopy in biology and medicine

Kachynski, Aliaksandr V. and Kuzmin, Andrey N. and Nyk, Marcin and Roy, Indrajit and Prasad, Paras N. (2008) Zinc oxide nanocrystals for nonresonant nonlinear optical microscopy in biology and medicine. JOURNAL OF PHYSICAL CHEMISTRY C, 112 (29). pp. 10721-10724.

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In this study, we showed that biocompatible zinc oxide (ZnO) nanocrystals (NCs) having a noncentrosymmetric structure can be used as nonresonant nonlinear optical probes for bioimaging applications in vitro by use of the second order processes of second harmonic and sum frequency generation, as well as the third order process of four wave mixing. These nonresonant processes provide advantages above and beyond traditional two photon bioimaging: (i) the probes do not photobleach; (ii) the input wavelength can be judiciously selected; and (iii) no heat is dissipated into the cells, ensuring longer cell viability and ultimately longer imaging times. ZnO NCs were synthesized in organic media by using a nonhydrolytic sol-gel process and subsequently dispersed in aqueous media using phospholipid micelles and incorporated with the biotargeting molecule folic acid (FA). Sum frequency, second harmonic, and nonresonant four wave mixing nonlinear signals from this stable dispersion of ZnO NCs, targeted to the live tumor (KB) cells, were used for imaging. Robust intracellular accumulation of the targeted (FA incorporated) ZnO nanocrystals could be observed without any indication of cytotoxicity.

Item Type:Article
Subjects:Material Science > Functional and hybrid materials
Biomedical Science > Nanobiotechnology
Material Science > Nanostructured materials
ID Code:1449
Deposited By:Farnush Anwar
Deposited On:12 Dec 2008 14:40
Last Modified:13 Jan 2009 16:40

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