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Refractive index maps and membrane dynamics of human red blood cells parasitized by Plasmodium falciparum

Park, YongKeun and Diez-Silva, Monica and Popescu, Gabriel and Lykotrafitis, George and Choi, Wonshik and Feld, Michael S. and Suresh, Subra (2008) Refractive index maps and membrane dynamics of human red blood cells parasitized by Plasmodium falciparum. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 105 (37). pp. 13730-13735.

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Official URL: http://www.pnas.org/content/105/37/13730.full.pdf+...

Abstract

Parasitization by malaria-inducing Plasmodium falciparum leads to structural, biochemical, and mechanical modifications to the host red blood cells (RBCs). To study these modifications, we investigate two intrinsic indicators: the refractive index and membrane fluctuations in A falciparum-invaded human RBCs (Pf-RBCs). We report experimental connections between these intrinsic indicators and pathological states. By employing two noninvasive optical techniques, tomographic phase microscopy and diffraction phase microscopy, we extract three-dimensional maps of refractive index and nanoscale cell membrane fluctuations in isolated RBCs. Our systematic experiments cover all intraerythrocytic stages of parasite development under physiological and febrile temperatures. These findings offer potential, and sufficiently general, avenues for identifying, through cell membrane dynamics, pathological states that cause or accompany human diseases.

Item Type:Article
Subjects:Biomedical Science > Nanoscale biological processes
Biomedical Science > Nanotechnology for human health
ID Code:134
Deposited By:Lesley Tobin
Deposited On:17 Nov 2008 16:39
Last Modified:30 Jan 2009 15:32

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