Spero, Richard Chasen and Vicci, Leandra and Cribb, Jeremy and Bober, David and Swaminathan, Vinay and O'Brien, E. Timothy and Rogers, Stephen L. and Superfine, R. (2008) High throughput system for magnetic manipulation of cells, polymers, and biomaterials. REVIEW OF SCIENTIFIC INSTRUMENTS, 79 (8).
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In the past decade, high throughput screening (HTS) has changed the way biochemical assays are performed, but manipulation and mechanical measurement of micro- and nanoscale systems have not benefited from this trend. Techniques using microbeads (particles similar to 0.1-10 mu m) show promise for enabling high throughput mechanical measurements of microscopic systems. We demonstrate instrumentation to magnetically drive microbeads in a biocompatible, multiwell magnetic force system. It is based on commercial HTS standards and is scalable to 96 wells. Cells can be cultured in this magnetic high throughput system (MHTS). The MHTS can apply independently controlled forces to 16 specimen wells. Force calibrations demonstrate forces in excess of 1 nN, predicted force saturation as a function of pole material, and powerlaw dependence of F similar to r(-2.7 +/- 0.1). We employ this system to measure the stiffness of SR2+ Drosophila cells. MHTS technology is a key step toward a high throughput screening system for micro- and nanoscale biophysical experiments. (C) 2008 American Institute of Physics.
|Uncontrolled Keywords:||biological techniques, biomagnetism, calibration, cellular biophysics, magnetic forces, polymers|
|Subjects:||Analytical Science > Microscopy and probe methods|
Material Science > Bio materials
Material Science > Soft materials
|Deposited By:||Lesley Tobin|
|Deposited On:||17 Nov 2008 16:48|
|Last Modified:||30 Jan 2009 16:30|
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