Funovic, Philip and Korda, Mykhaylo and Kubant, Ruslan and Barlag, Rebecca E. and Jacob, Robert F. and Mason, R. Preston and Malinski, Tadeusz (2008) Effect of beta-blockers on endothelial function during biological aging: A nanotechnological approach. JOURNAL OF CARDIOVASCULAR PHARMACOLOGY, 51 (2). pp. 208-215.
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Official URL: http://www.cardiovascularpharm.com/pt/re/jcardioph...
Biological aging is an independent risk factor for many cardiovascular diseases; some are treated with beta-blockers that may protect dysfunctional endothelium during aging by increasing NO, decreasing ONOO-, and restoring NO/ONOO- balance. A nanotechnological approach was used to simultaneously monitor NO and ONOO- produced by a single aortic endothelial cell from Wistar-Kyoto rats of different ages. beta-blockers (metoprolol and atenolol) were administered 2 weeks before the animals were sacrificed. Nanosensors were placed near the endothelium, and calcium ionophore-stimulated NO and ONOO- release was measured. Endothelial nitric oxide synthase (eNOS) undergoes uncoupling with aging, manifested by a decrease in NO (from 503 +/- 12 to 163 +/- 5 nmol/L) and a 3-fold increase in ONOO- for 16-week-old and 110- week- old rats, respectively. Metoprolol reversed eNOS uncoupling, increased the production rate and concentration of NO, and increased an overall ratio of NO]/ONOO-] This effect was not observed with atenolol, but L-arginine, sepiapterin, and superoxide dismutase were beneficial.
|Uncontrolled Keywords:||biological aging; nitric oxide; peroxynitrite; beta-blockers; endothelial nitric oxide synthase; L-arginine; superoxide demutase; nanosensors|
|Subjects:||Analytical Science > Nanotechnology for sensing and actuating|
Biomedical Science > Nanomedicine
|Deposited By:||Anuj Seth|
|Deposited On:||15 Dec 2008 15:45|
|Last Modified:||19 Jan 2009 11:03|
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