Nano Archive

Dielectrophoretic assembly of insulinoma cells and fluorescent nanosensors into three-dimensional pseudo-islet constructs

Pethig, R. and Menachery, A. and Heart, E. and Sanger, R. H. and Smith, P. J. S. (2008) Dielectrophoretic assembly of insulinoma cells and fluorescent nanosensors into three-dimensional pseudo-islet constructs. IET NANOBIOTECHNOLOGY, 2 (2). pp. 31-38.

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Abstract

Dielectrophoretic forces, generated by radio-frequency voltages applied to micromachined, transparent, indium tin oxide electrodes, have been used to condense suspensions of insulinoma cells (BETA-TC6 and INS-1) into a 10 x 10 array of three-dimensional cell constructs. Some of these constructs, measuring similar to 150 mu m in diameter, 120 mu m in height and containing around 1000 cells, were of the same size and cell density as a typical islet of Langerhans. With the dielectrophoretic force maintained, these engineered cell constructs were able to withstand mechanical shock and fluid flow forces. Reproducibility of the process required knowledge of cellular dielectric properties, in terms of membrane capacitance and membrane conductance, which were obtained by electrorotation measurements. The ability to incorporate fluorescent nanosensors, as probes of cellular oxygen and pH levels, into these `pseudo- islets' was also demonstrated. The footprint of the 10 x 10 array of cell constructs was compatible with that of a 1536 microtitre plate, and thus amenable to optical interrogation using automated plate reading equipment.

Item Type:Article
Subjects:Material Science > Functional and hybrid materials
Analytical Science > Nanotechnology for sensing and actuating
Biomedical Science > Nanobiotechnology
Material Science > Bio materials
ID Code:1230
Deposited By:Anuj Seth
Deposited On:16 Dec 2008 10:29
Last Modified:20 Jan 2009 10:06

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