Sheu, J. -T. and Chen, C. C. and Chang, K. S. and Li, Y. -K. (2008) A possibility of detection of the non-charge based analytes using ultra-thin body field-effect transistors. BIOSENSORS & BIOELECTRONICS, 23 (12). pp. 1883-1886.
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Official URL: http://dx.doi.org/10.1016/j.bios.2008.02.024
Ultra-thin body of p-type field-effect transistors were developed as transducer for biosensors. Changes of conductance resulted from the changes of the surface potentials of ultra-thin body field-effect transistors (UTB-FETs) due to surface chemical modifications were demonstrated. The channel surface of UTB-FETs were modified with N-[3-(trimethoxysilyl)propyl]ethylenediamine (AEAPTMS) and then gold nanoparticles (AuNPs) to immobilize the bio-component, the genetically engineered Delta(5)-3-ketosteroid isomerase (Art_KSI) or the Art_KSI conjugated with charged reporter (Art_KSI_mA51). The binding of charge-based molecules or nanoparticles has been demonstrated to strongly affect the conductivity of UTB-FETs: the increase or decrease of the conductance depends on the polarity of the immobilized molecules or nanoparticles. A new protocol involving the detection of a non-charged analyte relied on the competitive binding of analyte (19-norandrostendione) and a charged reporter (mA51) with KSL When exposed to a 19-norandrostendione solution (10 mu M), the conductance of Art_KSI_mA51-modified UTB-FET increased by 265 nS (similar to 12%). On the other hand, conductance of Art_KSI_modified UTB-FET showed no distinct change under the same detection conditions. (c) 2008 Elsevier B.V. All rights reserved
|Uncontrolled Keywords:||biosensors; ultra-thin body field-effect transistor; N-[3(trimethoxysilyl)propyl]ethylenediamine; gold nanoparticles; Delta(5)-3-ketosteroid isomerase; 19-norandrostendione|
|Subjects:||Analytical Science > Nanotechnology for sensing and actuating|
Material Science > Nanochemistry
Material Science > Nanostructured materials
|Deposited By:||Anuj Seth|
|Deposited On:||16 Dec 2008 10:41|
|Last Modified:||19 Jan 2009 11:30|
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