Nano Archive

Nanomonitors: electrical immunoassays for protein biomarker profiling

Bothara, Manish G. and Venkatraman, V and Reddy, R. K. K. and Barrett, Thomas W. and Carruthers, John and Prasad, S (2008) Nanomonitors: electrical immunoassays for protein biomarker profiling. NANOMEDICINE, 3 (4). pp. 423-436.

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Aims: The objective of this research is to develop a `point-of-care' device for early disease diagnosis through protein biomarker characterization. Here, we present label-free, high sensitivity detection of proteins with the use of electrical immunciassays that we call nanomonitors. Materials & methods: The basis of the detection principle lies in the formation of an electrical double layer and its perturbations caused by proteins trapped in a nanoporous alumina membrane over a microelectrode array platform. Results & discussion: High sensitivity and rapid detection of study protein biomarkers for cardiovascular diseases, C-reactive protein (CRP) and myeloperoxidase (MPO) in pure and clinical samples through label-free electrical detection were achieved. CRP and MPO were detected in pure solutions with a lower detection limit of 200 pg/ml and 500 pg/ml, respectively. These two study proteins were also detected from multiplexed samples containing a mixture of both proteins as well as human serum samples. Conclusions: The performance parameters of the nanomonitors, such as speed of detection on the order of minutes, volume of reagents of a few microliters and low cost per assay are comparable to traditional assay methods, such as ELISA. In addition, nanomonitors also provide the advantages of being a label-free technique with large linear dynamic range of detection and a significant reduction in the size of assay, thus making it an ideal candidate for a clinical diagnostic `lab-on-a-chip' device for protein biomarker profiling and hence early disease diagnosis.

Item Type:Article
Uncontrolled Keywords:biosensors; impedance spectroscopy; multiplexed detection; nanomembrane; protein
Subjects:Analytical Science > Nanotechnology for sensing and actuating
Biomedical Science > Nanobiotechnology
Biomedical Science > Nanotechnology for human health
Material Science > Nanostructured materials
Biomedical Science > Nanomedicine
ID Code:1215
Deposited By:Anuj Seth
Deposited On:16 Dec 2008 12:02
Last Modified:18 Feb 2009 17:55

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