Nano Archive

Oxygen microscopy by two-photon-excited phosphorescence

Finikova, Olga S. and Lebedev, Artem Y. and Aprelev, Alexey and Troxler, Thomas and Gao, Feng and Garnacho, Carmen and Muro, Silvia and Hochstrasser, Robin M. and Vinogradov, Sergei A. (2008) Oxygen microscopy by two-photon-excited phosphorescence. CHEMPHYSCHEM, 9 (12). pp. 1673-1679.

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High-resolution images of oxygen distributions in microheterogeneous samples are obtained by two-photon loser scanning microscopy (2P LSM), using a newly developed dendritic nanoprobe with internally enhanced two-photon absorption (2PA) cross-section. In this probe, energy is harvested by a 2PA antenna, which posses excitation onto a phosphorescent metalloporphyrin via intramolecular energy transfer. The 2P LSM allows sectioning of oxygen gradients with near diffraction-limited resolution, and life-time-based acquisition eliminates dependence on the local probe concentration. The technique is validated on objects with a priori known oxygen distributions and applied to imaging of pO(2) in cells.

Item Type:Article
Uncontrolled Keywords:dendrimer; energy transfer; oxygen; phosphorescence; porphyrin
Subjects:Analytical Science > Microscopy and probe methods
Biomedical Science > Nanobiotechnology
Material Science > Nanochemistry
ID Code:1211
Deposited By:Anuj Seth
Deposited On:16 Dec 2008 13:48
Last Modified:19 Jan 2009 11:55

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