Perez, J. Manuel and Grimm, Jan and Josephson, Lee and Weissleder, Ralph (2008) Integrated nanosensors to determine levels and functional activity of human telomerase. NEOPLASIA, 10 (10). pp. 1066-1072.
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Official URL: http://www.neoplasia.com/abstract.php?msid=1633
Telomerase is a key oncogenic enzyme, and a number of novel telomerase inhibitors are currently under development. Because inhibition can be achieved either at the protein or at the enzymatic activity level, independent measurements of these parameters are important in the development of effective therapeutic agents. In the current study, we have developed a set of functional magnetic nanosensors capable of measuring the concentration of telomerase, as well as its enzymatic activity in parallel. The method is based on a magnetic relaxation switch assay, which can be performed in crude tissue samples and is fast and extremely sensitive. Using this method, we were able to detect different amounts of telomerase protein and activity in various cancer and normal cell lines. Furthermore, we were able to study the effect of phosphorylation on telomerase activity. This system not only could provide a rapid assay for the evaluation of antitelomerase therapies but could also be implemented to the study of other cancer markers.
|Subjects:||Analytical Science > Nanotechnology for sensing and actuating|
Physical Science > Nanomagnetics
Biomedical Science > Nanotechnology for human health
Biomedical Science > Nanomedicine
|Deposited By:||Anuj Seth|
|Deposited On:||16 Dec 2008 14:16|
|Last Modified:||19 Jan 2009 12:05|
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