Nano Archive

Internalisation of polymeric nanosensors in mesenchymal stem cells: Analysis by flow cytometry and confocal microscopy

Coupland, Paul G. and Fisher, Karen A. and Jones, D. Rhodri E. and Aylott, Jonathan W. (2008) Internalisation of polymeric nanosensors in mesenchymal stem cells: Analysis by flow cytometry and confocal microscopy. JOURNAL OF CONTROLLED RELEASE, 130 (2). pp. 115-120.

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Official URL: http://dx.doi.org/10.1016/j.jconrel.2008.06.018

Abstract

The aim of this study was to demonstrate that flow cytometry and confocal microscopy could be applied in a complementary manner to analyse the internalisation of polymeric nanosensors in mesenchymal stem cells (MSC). The two techniques are able to provide en masse data analysis of nanosensors from large cell populations and detailed images of intracellular nanosensor localisation, respectively. The polyacrylamide nanosensors used in this investigation had been modified to contain free amine groups which were subsequently conjugated to Tat peptide, which acted as a delivery vector for nanosensor internalisation. Flow cytometry was used to confirm the health of MSC culture and assess the impact of nanosensor internalisation. MSC were characterised using fluorescently tagged CD cell surface markers that were also used to show that nanosensor internalisation did not negatively impact on MSC culture. Additionally it was shown that flow cytometry can be used to measure fluorophores located both on the cell surface and internalised within the cell. Complementary data was obtained using confocal microscopy to confirm nanosensor internalisation within MSC. (C) 2008 Elsevier B.V. All rights reserved.

Item Type:Article
Subjects:Analytical Science > Microscopy and probe methods
Analytical Science > Nanotechnology for sensing and actuating
Biomedical Science > Nanobiotechnology
Material Science > Nanostructured materials
ID Code:1196
Deposited By:Anuj Seth
Deposited On:16 Dec 2008 14:29
Last Modified:19 Jan 2009 12:09

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