Saengkrit, Nattika and Rakkhithawatthana , Given Name/Initials Varaporn and Rakkhithawatthana , Varaporn and Tencomnao, Tewin (2011) Nano-polyplex as a non-viral gene carrier for the expression of bone morphogenetic protein in osteoblastic cells. sciencedirect, 86 (2). pp. 587-583. ISSN 0144-8617
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The nano-polyplexes based gene delivery using the combination of water-soluble chitosan and polyethyleneimine (PEI) previously showed synergistic effect on gene transfection in a variety of human cell lines. This system simply facilitates gene therapy via a non-viral gene delivery system. Here, we have started to apply this system for therapeutic gene delivery into the osteoblastic-like cell line, MG-63. The transfection efficiency and cytotoxicity in MG-63 were investigated, intracellular uptake of nano-polyplexe was confirmed through confocal laser scanning microscope. Finally, bone morphogenesis protein (BMP) gene delivery was performed using our developed transfection system. The expression level of BMP-2 therapeutic gene was measured via reverse transcriptase polymerase chain reaction (RT-PCR). Comparing with commercial available system, lipofectamine, the expression of BMP were prolonged. The chitosan-PEI blend polyplex studied here showed the advantages in the aspects of high gene transfection with low cytoxicity into MG-63 cells. This study proposes the non-viral vector as a promising approach for bone regeneration though gene therapy. (C) 2011 Elsevier Ltd. All rights reserved.
|Subjects:||Physical Science > Nanophysics|
|Deposited On:||21 May 2012 17:00|
|Last Modified:||21 May 2012 17:00|
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