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In vitro toxicity assessment of three hydroxylated fullerenes in human skin cells

Saathoff, John G and Inman, Alfred O and Xia, Xin R and Riviere, Jim E and Monteiro-Riviere, Nancy A (2011) In vitro toxicity assessment of three hydroxylated fullerenes in human skin cells. Toxicology in Vitro .

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Carbon fullerenes possess unique properties and their interactions with biomolecules have widespread applications. Functionalization of fullerenes with hydroxyl groups (fullerenols) can increase the solubility and potential for cellular interaction, but the health and safety effects of varying degrees of fullerene hydroxylation in biological systems is poorly understood. Existing reports regarding the toxicity and inflammatory potential of fullerenols give conflicting conclusions. To further elucidate the potential for toxicity of fullerenols, human epidermal keratinocytes (HEK) were exposed to fullerenols (low (C60(OH)20), medium (C60(OH)24), and high (C60(OH)32)) at concentrations ranging from 0.000544–42.5 µg/ml for 24 and 48 h. A statistically significant (p < 0.05) decrease in viability with alamar Blue (aB) was noted only with C60(OH)32 at 42.5 µg/ml after 24 h. Nanoparticle (NP) controls showed minimal NP/assay interference of the three fullerenols with the aB viability assay. Normalized IL-8 concentration for C60(OH)20 was not significantly different from control, while C60(OH)24 and C60(OH)32 showed a significant decrease at 24 and 48 h. These results suggest that different hydroxylation of fullerenes caused no cytotoxicity or inflammation up to 8.55 µg/ml. These findings suggest that extrapolation across similar NP will be dependent upon surface chemistry and concentration which may affect the degree of agglomeration and thus biological effects.

Item Type:Article
Subjects:NanoSafety > Environment, health and safety aspects of nanotechnology
ID Code:11800
Deposited By:Prof Nancy Monteiro-Riviere
Deposited On:02 Mar 2012 20:07
Last Modified:02 Mar 2012 20:07

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