Zhang, Leshuai W and Zeng, Liling and Barron, Andrew R and Monteiro-Riviere, Nancy A (2007) Biological Interactions of Functionalized Single-Wall Carbon Nanotubes in Human Epidermal Keratinocyt. International Journal of Toxicology .
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Carbon nanotube–based nanovectors, especially functionalized nanotubes, have shown potential for therapeutic drug delivery. 6-Aminohexanoic acid–derivatized single-wall carbon nanotubes (AHA-SWNTs) are soluble in aqueous stock solutions over a wide range of physiologically relevant conditions; however, their interactions with cells and their biological compatibility has not been explored. Human epidermal keratinocytes (HEKs) were dosed with AHA-SWNTs ranging in concentration from 0.00000005 to 0.05 mg/ml. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) cell viability decreased significantly (p < 0.05) from 0.00005 to 0.05 mg/ml after 24 h. The proinflam- matory mediators of inflammation cytokines interleukin (IL)-6, IL-8, tumor necrosis factor (TNF)-α, IL-10, and IL-1β were also assessed. Cytokine analysis did not show a significant increase in IL-6 and IL-8 in the medium containing 0.000005 mg/ml of AHA-SWNTs from 1 to 48 h. IL-6 increased in cells treated with 0.05 mg/ml of AHA-SWNTs from 1 to 48 h, whereas IL-8 showed a significant increase at 24 and 48 h. No significant difference (p < .05) was noted with TNF-α, IL-10, and IL-1β expression at any time point. Transmission electron microscopy of HEKs treated with 0.05 mg/ml AHA-SWNTs for 24 h depicted AHA-SWNTs localized within intracytoplasmic vacuoles in HEKs. Treatment with the surfactant 1% Pluronic F127 caused dispersion of the AHA-SWNT aggregates in the culture medium and less toxicity. These data showed that the lower concentration of 0.000005 mg/ml of AHA-SWNTs maintains cell viability and induces a mild cytotoxicity, but 0.05 mg/ml of AHA-SWNTs demonstrated an irritation response by the increase in IL-8.
|Subjects:||Risk > Environment, health and safety aspects of nanotechnology|
|Deposited By:||Prof Nancy Monteiro-Riviere|
|Deposited On:||02 Mar 2012 16:39|
|Last Modified:||02 Mar 2012 16:39|
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