Nano Archive

Characteristics of functionalized nano-hydroxyapatite and internalization by human epithelial cell

Yan-zhong, Zhao and Yan-yan, Huang and Jun, Zhu and Shai-hong, Zhu and Zhi-you, Li and Ke-chao, Zhou (2011) Characteristics of functionalized nano-hydroxyapatite and internalization by human epithelial cell. NANOSCALE RESEARCH LETTERS, 6 (1). pp. 1-8. ISSN 1931-7573 (Print) 1556-276X (Online)

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Official URL: http://www.nanoscalereslett.com/content/6/1/600

Abstract

Hydroxyapatite is the main inorganic component of biological bone and tooth enamel, and synthetic hydroxyapatite has been widely used as biomaterials. In this study, a facile method has been developed for the fabrication of arginine-functionalized and europium-doped hydroxyapatite nanoparticles (Arg-Eu-HAP). The synthesized nanoparticles characterized by transmission electron microscopy, X-ray diffractometry, Fourier transform infrared, and Zeta potential analyzer. Its biological properties with DNA binding, cell toxicity, cell binding and intracellular distribution were tested by agarose gel electrophoresis assay, flow cytometry, and fluorescence microscope and laser scanning confocal microscope. The synthesized Arg-Eu-HAP could effectively bind DNA without any cytotoxicity and be internalized into the cytoplasm and perinuclear of human lung epithelial cells.

Item Type:Article
Subjects:Biomedical Science > Nanoscale biological processes
Biomedical Science > Nanotechnology for human health
ID Code:11589
Deposited By:M T V
Deposited On:05 Jan 2012 10:27
Last Modified:05 Jan 2012 10:27

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