Chiang, Chung-Jen and Lin, Li-Jen and Chen, Chih-Jung (2011) Caleosin-based nanoscale oil bodies for targeted delivery of hydrophobic anticancer drugs. Journal of Nanoparticle Research, 13 (12). pp. 7127-7137.
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Nanoscale artificial oil bodies (NOBs) could be assembled from plant oil, phospholipids (PLs), and oleosin (Ole) as previously reported. NOBs have a lipid-based structure that contains a central oil space enclosed by a monolayer of Ole-bound PLs. As an oil structural protein, Ole functions to maintain the integrity of NOBs. Like Ole, caleosin (Cal) is a plant oil-associated protein. In this study, we investigated the feasibility of NOBs assembled by Cal for targeted delivery of drugs. Cal was first fused with anti-HER2/ neu affibody (ZH2), and the resulting fusion gene (Cal–ZH2) was then expressed in Escherichia coli . Consequently, NOBs assembled with the fusion protein were selectively internalized by HER2/ neu -positive tumor cells. The internalization efficiency could reach as high as 90%. Furthermore, a hydrophobic anticancer drug, Camptothecin (CPT), was encapsulated into Cal-based NOBs. These CPT-loaded NOBs had a size around 200 nm and were resistant to hemolysis. Release of CPT from NOBs at the non-permissive condition followed a sustained and prolonged profile. After administration of the CPT formulation, Cal–ZH2-displayed NOBs exhibited a strong antitumor activity toward HER2/ neu -positive cells both in vitro and in vivo . The result indicates the potential of Cal-based NOBs for targeted delivery of hydrophobic drugs.
|Deposited By:||Prof. Alexey Ivanov|
|Deposited On:||05 Jan 2012 09:29|
|Last Modified:||05 Jan 2012 09:42|
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