Kaushik, Ajeet and Solanki, Pratima Rathee and Ansari, Anees Ahmad and Ahmad, Sharif and Malhotra, Bansi Dhar (2009) A nanostructured cerium oxide film-based immunosensor for mycotoxin detection. Nanotechnology, 20 (5). 055105.
Full text is not hosted in this archive but may be available via the Official URL, or by requesting a copy from the corresponding author.
Official URL: http://stacks.iop.org/0957-4484/20/i=5/a=055105
Rabbit-immunoglobulin antibodies (r-IgGs) and bovine serum albumin (BSA) have been immobilized onto solâgel-derived nanostructured cerium oxide (nanoCeO 2 ) film fabricated onto an indiumâtin-oxide (ITO) coated glass plate to detect ochratoxin-A (OTA). Broad reflection planes obtained in x-ray diffraction (XRD) patterns reveal the formation of CeO 2 nanostructures. Electrochemical studies reveal that nanoCeO 2 particles provide an increased electroactive surface area for loading of r-IgGs with desired orientation, resulting in enhanced electron communication between r-IgGs and electrode. BSA/r-IgGs/nano CeO 2 /ITO immunoelectrode exhibits improved characteristics such as linear range (0.5â6 ng dl â1 ), low detection limit (0.25 ng dl â1 ), fast response time (30 s) and high sensitivity (1.27 ÂµA ng â1 dl â1 cm â2 ). The high value of the association constant ( K a , 0.9 Ã 10 11 l mol â1 ) indicates the high affinity of the BSA/r-IgGs/nano CeO 2 /ITO immunoelectrode to OTA.
|Deposited By:||Prof. Alexey Ivanov|
|Deposited On:||02 Nov 2011 00:15|
|Last Modified:||02 Nov 2011 00:47|
Repository Staff Only: item control page