Nano Archive

A novel nanostructured iron oxide–gold bioelectrode for hydrogen peroxide sensing

Thandavan, Kavitha and Gandhi, Sakthivel and Sethuraman, Swaminathan and Rayappan, John Bosco Balaguru and MaheswariKrishnan, Uma (2011) A novel nanostructured iron oxide–gold bioelectrode for hydrogen peroxide sensing. Nanotechnology, 22 (26). p. 265505.

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Fe 3 O 4 nanoparticles covalently linked to a gold electrode have been used for immobilizing catalase (CAT) enzyme to sense the presence of various concentrations of H 2 O 2 . These nanoparticles ranging from 20 to 30 nm were synthesized by thermal co-precipitation of ferric and ferrous chlorides. SEM and XRD have been used for morphological and structural characterization of Fe 3 O 4 nanoparticles. CAT enzyme was linked covalently to the surface of iron oxide using carbodiimide in phosphate buffer (pH 7.4) at 4 °C. The enzyme–iron oxide link was confirmed by FT-IR spectroscopy. Sensing studies carried out using cyclic voltammetry showed a linear response of the CAT/nano Fe3O4 / Au bioelectrode towards H2O2 between 1.5 and 13.5 µM with a very sharp response time of 2 s.

Item Type:Article
ID Code:11270
Deposited By:Prof. Alexey Ivanov
Deposited On:01 Nov 2011 23:31
Last Modified:02 Nov 2011 00:47

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