Nano Archive

DNA interaction studies of new nano metal based anticancer agent: validation by spectroscopic methods

Tabassum, Sartaj and Sharma, Girish Chandra and Arjmand, Farukh and Azam, Ameer (2010) DNA interaction studies of new nano metal based anticancer agent: validation by spectroscopic methods. Nanotechnology, 21 (19). p. 195102.

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A new nano dimensional heterobimetallic Cu–Sn containing complex as a potential drug candidate was designed, synthesized and characterized by analytical and spectral methods. The electronic absorption and electron paramagnetic resonance parameters of the complex revealed that the Cu(II) ion exhibits a square pyramidal geometry with the two pyrazole nitrogen atoms, the amine nitrogen atom and the carboxylate oxygen of the phenyl glycine chloride ligand located at the equatorial sites and the coordinated chloride ion occupying an apical position. 119 Sn NMR spectral data showed a hexa-coordinated environment around the Sn(IV) metal ion. TEM, AFM and XRD measurements illustrate that the complex could induce the condensation of CT-DNA to a particulate nanostructure. The interaction of the Cu–Sn complex with CT-DNA was investigated by UV–vis absorption and emission spectroscopy, as well as cyclic voltammetric measurements. The results indicated that the complex interacts with DNA through an electrostatic mode of binding with an intrinsic binding constant K b = 8.42 × 10 4 M − 1 . The Cu–Sn complex exhibits effective cleavage of pBR322 plasmid DNA by an oxidative cleavage mechanism, monitored at different concentrations both in the absence and in the presence of reducing agents.

Item Type:Article
ID Code:11232
Deposited By:Prof. Alexey Ivanov
Deposited On:01 Nov 2011 23:29
Last Modified:02 Nov 2011 00:47

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