Li, Peng and Sherry, Alexander J. and Cortes, Jairo A. and Anagnostopoulos, Constantine and Faghri, Mohammad (2011) A blocking-free microfluidic fluorescence heterogeneous immunoassay for point-of-care diagnostics. BIOMEDICAL MICRODEVICES, 13 (3). pp. 475-483. ISSN 1387-2176 (Print) 1572-8781 (Online)
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Official URL: http://www.springerlink.com/content/u74l218782hl24...
In this article, a rapid, sensitive, and disposable microfluidic immunosensor is presented for point-of-care (POC) testing and clinical diagnosis. For the first time, the blocking process is eliminated from a microfluidic heterogeneous immunoassay by using protein A functionalized polydimethylsiloxane microchannels. The nonspecific binding of the assay is maintained around the chip background level by using a pair of antibodies with different affinity to protein A under optimized experimental conditions. C-reactive protein (CRP), a biomarker for inflammation and cardiovascular disease risk assessment, is selected as a model analyte to demonstrate the sensitivity of this blocking-free microfluidic heterogeneous immunoassay. A four parameter logistic function is used to model and assess the data. The limit of detection obtained is 0.54Â Î¼g/mL, which is lower than the cut-off value for clinical diagnosis. The overall assay is completed in 5Â min. The protein A modified PDMS chips wet-stored at 4Â°C can maintain biofunctionality up to 14Â months. The developed blocking-free microfluidic heterogeneous immunoassay will immediately provide benefits to most immunosensing microdevices targeted for POC diagnostics by shortening analysis time, simplifying fluid transportation, reducing sample consumption, and lowering waste generation.
|Uncontrolled Keywords:||Microfluidic immunoassay – Blocking-free – Fluorescence detection – C-reactive protein – Point-of-care diagnostics – Poly(dimethylsiloxane)|
|Subjects:||Biomedical Science > Nanobiotechnology|
Biomedical Science > Nanomedicine
|Deposited On:||08 Jun 2011 11:06|
|Last Modified:||08 Jun 2011 11:06|
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