Nano Archive

Erratum to: An integrated disposable device for DNA extraction and helicase dependent amplification

Mahalanabis, Madhumita and Do, Jaephil and ALMuayad, Hussam and Zhang, Jane Y. and Klapperich, Catherine M. (2011) Erratum to: An integrated disposable device for DNA extraction and helicase dependent amplification. BIOMEDICAL MICRODEVICES, 13 (3). pp. 599-602. ISSN 1387-2176 (Print) 1572-8781 (Online)

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In the original manuscript, we reported the demonstration of an integrated microfluidic chip that performed helicase dependent amplification (HDA) on samples containing live bacteria. Bacterial lysis, nucleic acid extraction, and DNA amplification with a fluorescent reporter were incorporated into a disposable polymer cartridge format. We reported that the device was able to detect as few as 10 colony-forming units (CFU) of E. coli in growth medium. While the main conclusions of the original paper remain sound, the data presented in support of those conclusions contained errors that we detail, discuss and correct here. In short, we misidentified a non-specific product as a specific product of our HDA reaction. We incorrectly called reactions containing the non-specific product (length 70 bp) positive. Further investigation demonstrated that our primer set was faulty and not capable of amplifying the specific product. Here we redesigned primers, sequenced all of the products and reran all of the experiments reported previously to generate a new, verified dataset.

Item Type:Article
Additional Information:Supplemental Materials:
Uncontrolled Keywords:DNA – Bacteria – Helicase dependent amplification – Isothermal amplification – Microfluidic chip – Plastic – Solid phase extraction
Subjects:Biomedical Science > Nanobiotechnology
Biomedical Science > Nanomedicine
ID Code:10707
Deposited By:SPI
Deposited On:08 Jun 2011 11:09
Last Modified:08 Jun 2011 11:09

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