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Lectin-mediated microfluidic capture and release of leukemic lymphocytes from whole blood

Vickers, Dwayne A. L. and Hincapie, Marina and Hancock, William S. and Murthy, Shashi K. (2011) Lectin-mediated microfluidic capture and release of leukemic lymphocytes from whole blood. BIOMEDICAL MICRODEVICES, 13 (3). pp. 565-571. ISSN 1387-2176 (Print) 1572-8781 (Online)

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Official URL: http://www.springerlink.com/content/u6666844484025...

Abstract

Lectins are a group of proteins that bind specifically and reversibly to mono- and oligosaccharide carbohydrate structures that are present on the surfaces of mammalian cells. The use of lectins as capture agents in microfluidic channels was examined with a focus on cells associated with T and B lymphocytic leukemia. In addition to examining the adhesion of Jurkat T and Raji B lymphocytes to a broad panel of lectins, this work also examined the capture of these cells from whole blood. Captured T and B lymphocytes were eluted from the microfluidic devices with a solution of the lectin’s inhibiting sugar. The capture and release steps were accomplished in under 1 h. The significance of this work lies within the realm of low-cost capture of abundant target cells with non-stimulatory elution capability.

Item Type:Article
Uncontrolled Keywords:Microfluidics – Lectins – Cell capture – Cell adhesion – Blood – Leukemia
Subjects:Biomedical Science > Nanobiotechnology
Biomedical Science > Nanomedicine
ID Code:10698
Deposited By:SPI
Deposited On:08 Jun 2011 11:13
Last Modified:08 Jun 2011 11:13

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