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A suction-type microfluidic immunosensing chip for rapid detection of the dengue virus

Weng, Chen-Hsun and Huang, Tze-Bin and Huang, Chih-Chia and Yeh, Chen-Sheng and Lei, Huan-Yao and Lee, Gwo-Bin (2011) A suction-type microfluidic immunosensing chip for rapid detection of the dengue virus. BIOMEDICAL MICRODEVICES, 13 (3). pp. 585-595. ISSN 1387-2176 (Print) 1572-8781 (Online)

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The enzyme-linked immunosorbent assay (ELISA) is widely used in medical diagnostics. In order to reduce the diagnosis time and to lower the consumption of sample/reagents in an ELISA assay, a suction-type, automatic, pneumatically-driven microfluidic chip has been designed and fabricated in this study. The microfluidic chip integrates a multi-functional micro-transport/mixing unit, for transporting metering and mixing of samples and reagents in order to automatically perform the entire ELISA protocol. A new surface modification has been adopted which allows for a high processing capacity. The detection sensitivity for the dengue virus is found to be 101 PFU/ml, which is much better than a conventional ELISA assay (103 PFU/ml). The entire assay time is only 30 min, which is much faster than with 96-well microtiter plates (4 h). The consumed sample and reagent volume is only 12 μl, which is less than a conventional assay (100 μl). The development of this microfluidic chip may be promising for other immunosensing applications.

Item Type:Article
Uncontrolled Keywords:Microfluidics – Suction – Immunosensing – ELISA – Dengue virus – Surface modification
Subjects:Biomedical Science > Nanobiotechnology
Biomedical Science > Nanomedicine
ID Code:10696
Deposited By:SPI
Deposited On:08 Jun 2011 11:14
Last Modified:08 Jun 2011 11:14

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