Nano Archive

Surface modification of permalloy (Ni80Fe20) nanoparticles for biomedical applications

Qin, Gaowu W. and Darain, Farzana and Wang, Hui and Dimitrov, Krassen (2011) Surface modification of permalloy (Ni80Fe20) nanoparticles for biomedical applications. Journal of Nanoparticle Research, 13 (1). pp. 45-51.

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Official URL: http://www.springerlink.com/content/j3513260027134...

Abstract

We report a simple and novel method for surface biofunctionalization onto recently reported Ni80Fe20 permalloy nanoparticles (~71 nm) and the immobilization of a model protein, IgG from human serum. The strategy of protein immobilization involved attachment of histidine-tagged streptavidin to the Ni80Fe20 nanoparticles via a non-covalent ligand binding followed by biotinylated human IgG binding on the nanoparticle surface using the specific high affinity avidin–biotin interaction. The biofunctionalization of Ni80Fe20 permalloy nanoparticles was confirmed by Fourier Transform InfraRed (FTIR) spectroscopy and protein denaturing gel electrophoresis (lithium dodecyl sulfate-polyacrylamide gel electrophoresis, LDS-PAGE). This protocol for surface functionalization of the novel nanometer-sized Ni80Fe20 permalloy particles with biological molecules could open diverse applications in disease diagnostics and drug delivery.

Item Type:Article
Subjects:Physical Science > Nanophysics
Physical Science > Nano objects
Material Science > Nanochemistry
Material Science > Nanostructured materials
Divisions:Faculty of Engineering, Science and Mathematics > School of Physics
Faculty of Engineering, Science and Mathematics > School of Chemistry
ID Code:10577
Deposited By:JNCASR
Deposited On:28 Feb 2011 06:35
Last Modified:28 Feb 2011 06:35

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