Yadav, Renu and Labhsetwar, Nitin and Kotwal, Swati and Rayalu, Sadhana (2011) Single enzyme nanoparticle for biomimetic CO2 sequestration. Journal of Nanoparticle Research, 13 (1). pp. 263-271.
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Official URL: http://www.springerlink.com/content/uv68w555723764...
Nanoparticle technology is being increasingly used in environmental sciences. We prepared single enzyme nanoparticle (SEN) by modifying the surface of carbonic anhydrase (CA) with a thin layer of organic/inorganic hybrid polymer. SEN-CA appears to be improving the stability of free enzyme. CA, as ubiquitously found enzyme, is involved in gaseous CO2 sequestration and is being looked as a promising candidate for combating global warming. We report here physical characterization of SEN-CA using transmission electron microscope (TEM), Fourier-transform infrared analysis (FTIR), X-ray diffraction analysis (XRD), and energy dispersive X-ray (EDX). Average size of SEN-CA particles appears to be in the range of 70-80 nm. We also report the effect of SEN formation on the kinetic parameters of free CA such as Michaelis-Menten constant (Km), maximum reaction velocity (Vmax), and storage stability of free CA and SEN-CA. The Vmax of SEN-CA (0.02857 mmol/min/mg) and free enzyme (0.02029 mmol/min/mg) is almost similar. Km has decreased from 6.143 mM for SEN-CA to 1.252 mM for free CA. The stabilization of CA by SEN formation results in improved the half-life period (up to 100 days). The formation of carbonate was substantiated by using gas chromatography (GC). The conversion of CO2 to carbonate was 61 mg of CaCO3/mg of CA and 20.8 mg of CaCO3/mg of CA using SEN-CA and free CA, respectively.
|Subjects:||Material Science > Functional and hybrid materials|
Material Science > Nanochemistry
|Divisions:||Faculty of Engineering, Science and Mathematics > School of Chemistry|
|Deposited On:||04 May 2011 07:34|
|Last Modified:||04 May 2011 07:34|
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