Nano Archive

3D microfilter device for viable circulating tumor cell (CTC) enrichment from blood

Zheng, Siyang and Lin, Henry K. and Lu, Bo and Williams, Anthony and Datar, Ram and Cote, Richard J. and Tai, Yu-Chong (2010) 3D microfilter device for viable circulating tumor cell (CTC) enrichment from blood. Biomedical Microdevices, 13 (1). pp. 203-213.

Full text is not hosted in this archive but may be available via the Official URL, or by requesting a copy from the corresponding author.

Abstract

Detection of circulating tumor cells has emerged as a promising minimally invasive diagnostic and prognostic tool for patients with metastatic cancers. We report a novel three dimensional microfilter device that can enrich viable circulating tumor cells from blood. This device consists of two layers of parylene membrane with pores and gap precisely defined with photolithography. The positions of the pores are shifted between the top and bottom membranes. The bottom membrane supports captured cells and minimize the stress concentration on cell membrane and sustain cell viability during filtration. Viable cell capture on device was investigated with scanning electron microscopy, confocal microscopy, and immunofluorescent staining using model systems of cultured tumor cells spiked in blood or saline. The paper presents and validates this new 3D microfiltration concept for circulation tumor cell enrichment application. The device provides a highly valuable tool for assessing and characterizing viable enriched circulating tumor cells in both research and clinical settings.

Item Type:Article
Subjects:Biomedical Science > Nanoscale biological processes
ID Code:10484
Deposited By:CSMNT
Deposited On:06 Jun 2011 08:45
Last Modified:06 Jun 2011 08:45

Repository Staff Only: item control page